Unit-V Protein and Gene Manipulation

Chapter-1: Recombinant DNA Technology

  • Introduction,
  • Tool of DNA technology
  • Making DNA
  • Introduction of recombinant DNA into¬†host cells
  • Identification of recombinants
  • Polymerase Chain Reaction (PCR)
  • Hybridization techniques
  • DNA library
  • DNA Sequencing
  • Site-directed Mutagenesis

Chapter-2: Protein Structure and Engineering

  • Introduction to the world of proteins
  • 3-D shape of proteins
  • Structure-function Relationship in proteins
  • Purification of proteins
  • Characterization of proteins
  • Protein based products
  • Designing proteins (Protein Engineering)

Chapter-3: Genomics, Proteomics and Bioinformatics

  • Introduction
  • Genome, Sequencing projects
  • Gene prediction and counting
  • Genome similarity
  • SNPs and Comparative genomics
  • Functional genomics
  • Proteomics
  • History of bioinformatics
  • Sequences and nomenclature
  • Information sources
  • Analysis using bioinformatics tools.

Unit-VI Cell Culture and Genetic Manipulation

Chapter-1: Microbial Cell Culture and its Applications

  • Introduction Microbial nutrition and culture techniques
  • Measurement and kinetics of microbial growth
  • Scale-up of microbial process
  • Isolation of microbial products
  • Strain isolation and improvement
  • Applications of microbial culture technology
  • Biosafety issues in microbial technology

Chapter -2: Plant Cell Culture and Applications

  • Introduction
  • Cell and tissue culture techniques
  • Applications of cell and tissue culture
  • Gene transfer Methods in plants
  • Transgenic plants with beneficial traits
  • Biosafety of transgenic plants

Chapter-3: Animal Cell Culture and Applications

  • Introduction
  • Animal cell culture techniques
  • Characterization of cell lines
  • Methods of gene delivery into cells
  • Scale-up of animal culture process
  • Applications of animal cell culture
  • Stem cell technology
  • Tissue engineering.